Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis

摘要: This report describes the development of in-house real-time PCR assays using minor groove binding probes for simultaneous detection Bacillus anthracis pag and cap genes, Francisella tularensis 23 KDa gene, as well Yersinia pestis pla gene. The sensitivities these were at least 1 fg, except assay targeting which showed a sensitivity 10 fg when total DNA was used template in serial dilution. clinical value anthracis- tularensis-specific demonstrated by successful amplification from cases cow anthrax hare tularemia, respectively. No cross-reactivity between species-specific or with 39 other bacterial species noted. These may provide rapid tool identification three category A listed biological threats Centers Disease Control Prevention.