Jagged-1-HES-1 signaling inhibits the differentiation of TH17 cells via ROR gammat.

摘要: Notch signaling plays an important role in differentiation of T cells. However, little is known as to action it Th17 cell subset. In this study, a soluble Jagged-1/Fc chimera protein (Jagged-1) was directly used activate Jagged-1-Notch signaling, while Hes-1-targeting siRNA knock down Hes-1 gene investigate effect Jagged-1-Hes-1 on the CD4+ cells into The results showed that Jagged-1 could promote expression and Deltex-1 mRNAs NICD, proteins, which might be significantly blocked by DAPT, specific inhibitor signaling. resulted markedly decreased situ RORgammat induced IL-6 plus TGF-s. Flow cytometric analysis reduction IL-17 production Jagged-1, but enhancement siRNA. level IL-10 produced treated also enhanced, whereas prominently attenuated, offset anti-Jagged-1 antibody or DAPT. indicate can suppress skewing toward via RORgammat, for may crucial.