The light spot test : Measuring anxiety in mice in an automated home-cage environment
作者： Emmeke Aarts , Gregoire Maroteaux , Maarten Loos , Bastijn Koopmans , Jovana Kovačević
DOI: 10.1016/J.BBR.2015.06.011
关键词:
摘要: Behavioral tests of animals in a controlled experimental setting provide valuable tool to advance understanding genotype-phenotype relations, and study the effects genetic environmental manipulations. To optimally benefit from increasing numbers genetically engineered mice, reliable high-throughput methods for comprehensive behavioral phenotyping mice lines have become necessity. Here, we describe development validation an anxiety test, light spot that allows unsupervised, automated, testing home-cage system. This automated test circumvents bias introduced by pretest handling, enables recording both baseline behavior response over prolonged period time. We demonstrate induces C57BL/6J mice. reverts when aversive stimulus is switched off, blunted treatment with anxiolytic drug Diazepam, demonstrating predictive validity assay, indicating observed has significant component. Also, investigated effectiveness as part sequential different aspects home-cage. Two learning tests, administered prior affected parameters. The novel, assay anxiety-related environment, allowing rapid screening pharmacological compounds.
core.ac.uk
narcis.nl
vumc.nl
elsevier.com参考文章(27)
Martien J. H. Kas, Annetrude (J. G.) de Mooij-van Malsen, Berend Olivier, Berry M. Spruijt, Jan M. van Ree, Differential genetic regulation of motor activity and anxiety-related behaviors in mice using an automated home cage task. Behavioral Neuroscience. ,vol. 122, pp. 769- 776 ,(2008) , 10.1037/0735-7044.122.4.769
Laurence H Tecott, Eric J Nestler, Neurobehavioral assessment in the information age Nature Neuroscience. ,vol. 7, pp. 462- 466 ,(2004) , 10.1038/NN1225
R. J. Rodgers, J. K. Shepherd, Influence of prior maze experience on behaviour and response to diazepam in the elevated plus-maze and light/dark tests of anxiety in mice Psychopharmacology. ,vol. 113, pp. 237- 242 ,(1993) , 10.1007/BF02245704
Arjan Blokland, Sanne ten Oever, Dennis van Gorp, Michael van Draanen, Theodor Schmidt, Emily Nguyen, Alexandra Krugliak, Anthony Napoletano, Sarah Keuter, Inge Klinkenberg, The use of a test battery assessing affective behavior in rats: Order effects Behavioural Brain Research. ,vol. 228, pp. 16- 21 ,(2012) , 10.1016/J.BBR.2011.11.042
Vootele Voikar, Giovanni Colacicco, Oliver Gruber, Elisabetta Vannoni, Hans-Peter Lipp, David P. Wolfer, Conditioned response suppression in the IntelliCage: assessment of mouse strain differences and effects of hippocampal and striatal lesions on acquisition and retention of memory. Behavioural Brain Research. ,vol. 213, pp. 304- 312 ,(2010) , 10.1016/J.BBR.2010.05.019
Toshihiro Endo, Fumihiko Maekawa, Vootele Võikar, Asahi Haijima, Yukari Uemura, Yan Zhang, Wataru Miyazaki, Shigetomo Suyama, Kuniko Shimazaki, David P. Wolfer, Toshihiko Yada, Chiharu Tohyama, Hans-Peter Lipp, Masaki Kakeyama, Automated test of behavioral flexibility in mice using a behavioral sequencing task in IntelliCage. Behavioural Brain Research. ,vol. 221, pp. 172- 181 ,(2011) , 10.1016/J.BBR.2011.02.037
Kiersten S. Smith, Elif Engin, Edward G. Meloni, Uwe Rudolph, Benzodiazepine-induced anxiolysis and reduction of conditioned fear are mediated by distinct GABAA receptor subtypes in mice. Neuropharmacology. ,vol. 63, pp. 250- 258 ,(2012) , 10.1016/J.NEUROPHARM.2012.03.001
Leonie de Visser, Ruud van den Bos, Berry M. Spruijt, Automated home cage observations as a tool to measure the effects of wheel running on cage floor locomotion. Behavioural Brain Research. ,vol. 160, pp. 382- 388 ,(2005) , 10.1016/J.BBR.2004.12.004
Dani Brunner, Eric Nestler, Emer Leahy, In need of high-throughput behavioral systems Drug Discovery Today. ,vol. 7, pp. S107- S112 ,(2002) , 10.1016/S1359-6446(02)02423-6
Sandra E. File, The interplay of learning and anxiety in the elevated plus-maze Behavioural Brain Research. ,vol. 58, pp. 199- 202 ,(1993) , 10.1016/0166-4328(93)90103-W