In vitro-generated respiratory mucosa: a new tool to study inhalational anthrax.
作者： Svetlana N. Radyuk , Patricia A. Mericko , Taissia G. Popova , Edith Grene , Ken Alibek
DOI: 10.1016/S0006-291X(03)00830-1
关键词:
摘要: Abstract We generated a three-dimensional (3-D) model of human airway tissues in order to study initiation inhalational form anthrax infection. The system was designed the air–blood barrier respiratory tract represented by epithelial cells and macrophages. When grown on collagen/fibronectin gel support at an air–liquid interface, formed cell layers morphologically resembling those vivo. These preformed cultures were further supplemented with monocytes/macrophages isolated from blood. After 2–5 days co-culture, monocytes differentiated into phenotype resident macrophages, which evaluated expression specific surface markers. This allowed sorting out role each type found air lung. interdependence macrophages clearance spores airways capacity protect infection demonstrated.
elsevier.com
sci-hub.se 参考文章(31)
N. K. Jaax, M. L. M. Pitt, D. L. Fritz, K. J. Davis, A. M. Friedlander, W. B. Lawrence, J. W. Ezzell, Pathology of experimental inhalation anthrax in the rhesus monkey Laboratory Investigation. ,vol. 73, pp. 691- 702 ,(1995)
I. Stříž, A. Slavčev, J. Kalanin, M. Jarešová, S. I. Rennard, Cell-cell contacts with epithelial cells modulate the phenotype of human macrophages. Inflammation. ,vol. 25, pp. 241- 246 ,(2001) , 10.1023/A:1010975804179
K. A. Birkness, M. Deslauriers, J. H. Bartlett, E. H. White, C. H. King, F. D. Quinn, An In Vitro Tissue Culture Bilayer Model To Examine Early Events in Mycobacterium tuberculosis Infection Infection and Immunity. ,vol. 67, pp. 653- 658 ,(1999) , 10.1128/IAI.67.2.653-658.1999
Michel M. van den Heuvel, Cornelis P. Tensen, Jaco H. van As, Timo K. van den Berg, Donna M. Fluitsma, Christine D. Dijkstra, Ed A. Döpp, Anne Droste, Floris A. van Gaalen, Clemens Sorg, Petra Högger, Rob H. J. Beelen, Regulation of CD 163 on human macrophages: cross-linking of CD163 induces signaling and activation. Journal of Leukocyte Biology. ,vol. 66, pp. 858- 866 ,(1999) , 10.1002/JLB.66.5.858
Nicolas Castillon, Jocelyne Hinnrasky, Jean-Marie Zahm, Hervé Kaplan, Noël Bonnet, Pascal Corlieu, Jean-Michel Klossek, Karima Taouil, Aurélie Avril-Delplanque, Bruno Péault, Edith Puchelle, Polarized Expression of Cystic Fibrosis Transmembrane Conductance Regulator and Associated Epithelial Proteins during the Regeneration of Human Airway Surface Epithelium in Three-Dimensional Culture Laboratory Investigation. ,vol. 82, pp. 989- 998 ,(2002) , 10.1097/01.LAB.0000022221.88025.43
Moshe Kalina, Shoshana Riklis, Alveolar type II-like cell colonies: effect of alveolar macrophages and macrophage-conditioned media. Mechanisms of Development. ,vol. 23, pp. 231- 236 ,(1988) , 10.1016/0045-6039(88)90076-0
Serguei G Popov, Rafael Villasmil, Jessica Bernardi, Edith Grene, Jennifer Cardwell, Aiguo Wu, Darya Alibek, Charles Bailey, Ken Alibek, Lethal toxin of Bacillus anthracis causes apoptosis of macrophages Biochemical and Biophysical Research Communications. ,vol. 293, pp. 349- 355 ,(2002) , 10.1016/S0006-291X(02)00227-9
Lev M Grinberg, Faina A Abramova, Olga V Yampolskaya, David H Walker, Jerome H Smith, Quantitative Pathology of Inhalational Anthrax I: Quantitative Microscopic Findings Modern Pathology. ,vol. 14, pp. 482- 495 ,(2001) , 10.1038/MODPATHOL.3880337
Neil R. Mathias, Julita Timoszyk, Paul I. Stetsko, John R. Megill, Ronald L. Smith, Doris A. Wall, Permeability characteristics of calu-3 human bronchial epithelial cells: in vitro-in vivo correlation to predict lung absorption in rats. Journal of Drug Targeting. ,vol. 10, pp. 31- 40 ,(2002) , 10.1080/10611860290007504
V. J. TORMEY, J. FAUL, C. LEONARD, C. M. BURKE, A. DILMEC, L. W. POULTER, T-cell cytokines may control the balance of functionally distinct macrophage populations Immunology. ,vol. 90, pp. 463- 469 ,(1997) , 10.1046/J.1365-2567.1997.00207.X