Cryopreserving Human Peripheral Blood Progenitor Cells
作者： Anne Bakken
DOI: 10.2174/157488806775269179
关键词:
摘要: UNLABELLED High-dose chemotherapy followed by autologous peripheral blood progenitor cell (PBPC) transplantation is used in the treatment of chemosensitive malignancies. Cryopreservation PBPC 10% dimethyl sulfoxide (DMSO) has been standard procedure most institutions. Infusion cryopreserved with DMSO can be associated toxic reactions such as vomiting, cardiac dysfunction, anaphylaxia and acute renal failure. The grade toxicity experienced patients related to amount present PBPC. lower concentrations would expected reduce toxicity. In recent studies done cells 5%, 4% 2% DMSO, using a reference control, CD34+ were investigated for preservation viability, apoptosis, necrosis. Also mature colony-forming (CFU) cells, specifically myeloid, erythroid progenitors, CFU-megakaryocytes long-term culture-initiating (LTC-ICs) investigated, 5% cryoprotectant. All samples frozen rate-controlled programmed freezer stored vapor phase liquid nitrogen until used. CONCLUSION optimal concentration cryopreserving human vitro. Consequently, some hospitals have started cryoprotectant procedure.
eurekaselect.com参考文章(43)
M Labopin, J H Antin, W Aird, N C Gorin, Long-term cryopreservation of human stem cells. Bone Marrow Transplantation. ,vol. 9, pp. 487- 490 ,(1992)
U Elkayam, S Lau, S C Chen, Y K Keung, D Douer, Cardiac arrhythmia after infusion of cryopreserved stem cells Bone Marrow Transplantation. ,vol. 14, pp. 363- 367 ,(1994)
C. Perotti, P. Pedrazzoli, G. Poggi, G. A. Da Prada, A. Cuomo, P. Preti, A. Zambelli, G. Robustellidella Cuna, D. Miotti, Clinical toxicity of cryopreserved circulating progenitor cells infusion Anticancer Research. ,vol. 18, pp. 4705- 4708 ,(1998)
SD Rowley, WI Bensinger, TA Gooley, CD Buckner, Effect of Cell Concentration on Bone Marrow and Peripheral Blood Stem Cell Cryopreservation Blood. ,vol. 83, pp. 2731- 2736 ,(1994) , 10.1182/BLOOD.V83.9.2731.2731
Taner Demirer, William I. Bensinger, C. Dean Buckner, Peripheral blood stem cell mobilization for high-dose chemotherapy. Journal of hematotherapy. ,vol. 8, pp. 103- 113 ,(1999) , 10.1089/106161299320389
D. ROBERT SUTHERLAND, LORI ANDERSON, MICHAEL KEENEY, RAKASH NAYAR, IAN CHIN-YEE, The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. Journal of hematotherapy. ,vol. 5, pp. 213- 226 ,(1996) , 10.1089/SCD.1.1996.5.213
Patricia A Denning-Kendall, JM Hows, AJ Nicol, C Donaldson, WJ Armitage, BA Bradley, Optimal cryopreservation of human umbilical cord blood. Bone Marrow Transplantation. ,vol. 18, pp. 725- 731 ,(1996)
Pierre Feugier, Daniele Bensoussan, Florence Girard, Francois Alla, Anne Schuhmacher, Veronique Latger-Cannard, Cyrille Hulin, Francis Witz, Brigitte Witz, Anne Sophie Carret, Pierre Bordigoni, Pierre Lederlin, Christian Janot, Hematologic recovery after autologous PBPC transplantation: importance of the number of postthaw CD34+ cells. Transfusion. ,vol. 43, pp. 878- 884 ,(2003) , 10.1046/J.1537-2995.2003.00446.X
A Humpe, J Riggert, K Vehmeyer, C Troff, W Hiddemann, M Kohler, B Wormann, Comparison of CD34+ cell numbers and colony growth before and after cryopreservation of peripheral blood progenitor and stem cell harvests: influence of prior chemotherapy Transfusion. ,vol. 37, pp. 1050- 1057 ,(1997) , 10.1046/J.1537-2995.1997.371098016444.X
DS Allan, M Keeney, K Howson-Jan, J Popma, K Weir, M Bhatia, DR Sutherland, IH Chin-Yee, Number of viable CD34(+) cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation. Bone Marrow Transplantation. ,vol. 29, pp. 967- 972 ,(2002) , 10.1038/SJ.BMT.1703575