In vitro exposure of a novel polyesterurethane graft to enzymes: a study of the biostability of the Vascugraft arterial prosthesis.

摘要: Abstract The biostability of the Vascugraft® arterial prosthesis, a porous synthetic graft made by novel spinning process from unique poly(ester urethane) polymer, has been studied means an in vitro enzyme incubation technique. Samples were exposed to buffered solutions collagenase and pancreatin, as well buffer alone, for periods up 100 days at 37 ± 1°C. On removal after cleaning, number different analytic methods, including X-ray photoelectron spectroscopy chemical analysis (ESCA), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC), electron microscopy (SEM), interference microscopy, moisture content contact angle measurements, used examine changes structure surface morphology samples. During both enzymes molecular weight polyurethane appeared decrease presence but increase buffer. Further microphase separation material developed during solutions. Such microstructure associated with increased hydrophilicity, significant improvement extent order preferred orientation hard segment domains within fibres. In sampling depth about 5 nm, decreased carbonate group prosthesis little 40% their original values. results ATR-FTIR DSC demonstrated that this phenomenon was limited primarily soft phase. While did exhibit some modifications on exposure concentrated solutions, nevertheless such confined layer microfibres. importance significance those will be more adequately determined vivo investigation.