Isolation and characterization of rhesus monkey milk lactoferrin.

摘要: Rhesus monkey milk lactoferrin was isolated and its characteristics compared with those of human in order to assess the feasibility using rhesus as an animal model for study iron absorption from milk. Monkey pooled by two chromatographic steps. Concentration milk, determined rocket immunoelectrophoresis, demonstrated similar concentrations both 1-2 mg/ml. Immunodiffusion lactoferrins several species antibody raised money resulted a cross-reaction only lactoferrin. Lactoferrins cow, sheep, goat, dog, rat were not recognized antibody. Amino acid analysis showed composition very lactoferrin, well similarity unusual amino sequence at N-terminal protein. The carbohydrate moiety investigated shown contain monosaccharides proportions reported In our opinion, is promising role infant, other proposed actions