Engineering of glucose oxidase for direct electron transfer via site-specific gold nanoparticle conjugation.
作者： J. Todd Holland , Carolin Lau , Susan Brozik , Plamen Atanassov , Scott Banta
DOI: 10.1021/JA2071237
关键词:
摘要: Optimizing the electrical communication between enzymes and electrodes is critical in development of biosensors, enzymatic biofuel cells, other bioelectrocatalytic applications. One approach to address this limitation attachment redox mediators or relays enzymes. Here we report a simple genetic modification glucose oxidase enzyme display free thiol group near its active site. This facilitates site-specific maleimide-modified gold nanoparticle enzyme, which enables direct conjugated an electrode. Glucose particular interest cell biosensor applications, "prewiring" conjugates manner will be valuable continued these systems.
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Veronica Blakeley, Kanjula D. Seneviratne, Michael J. McPherson, Peter F. Knowles, Simon E V Phillips, Conrad Stevens, Carrie Wilmot, Andrew J. Baron, David M. Dooley, Structure and mechanism of galactose oxidase. The free radical site. Journal of Biological Chemistry. ,vol. 269, pp. 25095- 25105 ,(1994) , 10.1016/S0021-9258(17)31504-1
Andrey L. Ghindilis, Plamen Atanasov, Ebtisam Wilkins, Enzyme‐catalyzed direct electron transfer: Fundamentals and analytical applications Electroanalysis. ,vol. 9, pp. 661- 674 ,(1997) , 10.1002/ELAN.1140090902
F. Battaglini, P. N. Bartlett, J. H. Wang, Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose Analytical Chemistry. ,vol. 72, pp. 502- 509 ,(2000) , 10.1021/AC990321X
P.N. Barlett, J.M. Cooper, A review of the immobilization of enzymes in electropolymerized films Journal of Electroanalytical Chemistry. ,vol. 362, pp. 1- 12 ,(1993) , 10.1016/0022-0728(93)80001-X
Fei Mao, Nicolas Mano, Adam Heller, Long tethers binding redox centers to polymer backbones enhance electron transport in enzyme "Wiring" hydrogels. Journal of the American Chemical Society. ,vol. 125, pp. 4951- 4957 ,(2003) , 10.1021/JA029510E
Wolfgang Schuhmann, Electron-transfer pathways in amperometric biosensors. Ferrocene-modified enzymes entrapped in conducting-polymer layers Biosensors and Bioelectronics. ,vol. 10, pp. 181- 193 ,(1995) , 10.1016/0956-5663(95)96805-9
Qijin Chi, Jingdong Zhang, Shaojun Dong, Erkang Wang, Direct electrochemistry and surface characterization of glucose oxidase adsorbed on anodized carbon electrodes Electrochimica Acta. ,vol. 39, pp. 2431- 2438 ,(1994) , 10.1016/0013-4686(94)00202-9
Lorena Betancor, Manuel Fuentes, Gisella Dellamora-Ortiz, Fernando López-Gallego, Aurelio Hidalgo, Noelia Alonso-Morales, Cesar Mateo, José M Guisán, Roberto Fernández-Lafuente, None, Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles Journal of Molecular Catalysis B-enzymatic. ,vol. 32, pp. 97- 101 ,(2005) , 10.1016/J.MOLCATB.2004.11.003
Frédéric Barrière, Paul Kavanagh, Dónal Leech, A laccase-glucose oxidase biofuel cell prototype operating in a physiological buffer Electrochimica Acta. ,vol. 51, pp. 5187- 5192 ,(2006) , 10.1016/J.ELECTACTA.2006.03.050
Philippe De Taxis Du Poet, Shigeyuki Miyamoto, Toru Murakami, Jun Kimura, Isao Karube, Direct electron transfer with glucose oxidase immobilized in an electropolymerized poly( N-methylpyrrole) film on a gold microelectrode Analytica Chimica Acta. ,vol. 235, pp. 255- 263 ,(1990) , 10.1016/S0003-2670(00)82082-6