Development and validation of an open source O2-sensitive gel for physiological profiling of soil microbial communities.
作者： E.S. McLamore , J.L. Garland , C. Mackowiak , A. Desaunay , N. Garland
DOI: 10.1016/J.MIMET.2013.10.016
关键词:
摘要: Abstract Community level physiological profiling is a simple, high-throughput technique for assessing microbial community physiology. Initial methods relying on redox-dye based detection of respiration were subject to strong enrichment bias, but subsequent development microtiter assay using an oxygen-quenched dye reduced this bias and improved the versatility approach. Commercial production oxygen microplates recently stopped, which led present effort develop validate system luminophore (platinum tetrakis pentafluorophenyl) immobilized at bottom wells within 96 well plate. The was used analyze three well-characterized Florida soils: oak saw palmetto scrub, coastal mixed hardwood, soil from agricultural field grow corn silage. Substrate induced monitored by measuring rates in soils under basal conditions comparing supplemented with nitrogen various carbon sources (mannose, casein, asparagine, coumaric acid). All data compared previously available commercial assay. There no significant differences maximum peak intensity or time response all tested (p
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