Effects of tillage and fertilization on physiological profiles of soil microbial communities
作者： E. Gomez , J.L. Garland
DOI: 10.1016/J.APSOIL.2011.10.008
关键词:
摘要: Abstract The present work evaluated the ability of a rapid physiological profiling method to determine long term effects alternative tillage practices (no till vs. chisel plow) and nitrogen fertilization on soil microbial activity. Community respiratory response (i.e., O 2 consumption) carbon mineral nutrient (N P) amendments was examined in samples (Typic Argiudoll) from N-fertilization trial. consumption assessed under control conditions C-substrate added) addition (50 μg ml −1 ) five sources: acetate, asparagine, coumaric acid, mannose, carboxy-methyl cellulose (CMC). effect limitation activity by single or combined N P (5.3 μg ml N 0.6 μg ml P as (NH 4 SO K HPO , respectively). High throughput analysis enabled automated monitoring an -quenched fluorophore immobilized gel added microtiter plates. Basal control) respiration caused 15–25% increase maximum peak fluorescence ( F max while 30–450% increases across all soils with CMC N + P assay. General not consistently observed except case acid use. However, plate varied depending treatment. Overall, -based CLPP approach effective for detecting management overall well shifts functional groups bioavailability P.
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