Using Synthetic Oligonucleotides as Probes
作者： Allan Duby , Kenneth A. Jacobs , Anthony Celeste
DOI: 10.1002/0471142727.MB0604S09
关键词:
摘要: The protocols in this unit describe procedures for using mixtures of 32P-labeled oligonucleotides to screen recombinant DNA clones bound nitrocellulose filters. A partial amino acid sequence a protein is used predict the nucleotide gene that would encode it. mixture chosen includes all possible sequences encoding sequence. This then library corresponding clones. In some cases however, exact desired clone known and it use unique oligonucleotide as probe.
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