Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata

摘要: Abstract The endophytic fungi, Pestalotiopsis versicolor and neglecta , were isolated from the healthy leaves bark of Japanese Yew tree, Taxus cuspidata . fungal species identified by their characteristic culture morphology molecular analysis. For first time, test fungi screened for production taxol in modified liquid medium. presence was confirmed HPLC, 1 H NMR, LC–MS methods maximum amount P. recorded as 478 μg/l. rate increased to 9560-fold than that found broth earlier reported fungus, Taxomyces andreanae extracted showed a strong cytotoxic activity vitro tested human cancer cells apoptotic assay indicating increase concentration induces cell death. A PCR-based screening ts unique gene formation taxane skeleton, blueprint biosynthesis. results designate endophyte, is an excellent candidate alternate source supply can also serve potential genetic engineering enhance higher level.