Investigation of the Essentiality of Glutamate Racemase in Mycobacterium smegmatis

摘要: The mycobacterial cell wall frequently has been used as a target for drug development, and d-glutamate, synthesized by glutamate racemase (MurI), is an important component of peptidoglycan. While the essentiality murI gene shown in several bacterial species, including Escherichia coli, Bacillus anthracis, Streptococcus pneumoniae, studies mycobacteria have not yet provided definitive results. This study aimed to determine whether indeed essential can serve possible structure-aided design. We achieved this goal creating ΔmurI strain Mycobacterium smegmatis, close relative tuberculosis. deletion M. smegmatis could be only minimal medium supplemented with demonstrating that MurI growth source d-glutamate peptidoglycan synthesis smegmatis.