MALAT1 promotes the proliferation and invasion of thyroid cancer cells via regulating the expression of IQGAP1
作者： Jian-kang Huang , Ling Ma , Wen-hua Song , Bang-yu Lu , Yu-bin Huang
DOI: 10.1016/J.BIOPHA.2016.05.039
关键词:
摘要: Abstract Background Increasing evidence indicated that metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) acted as a key regulator in the proliferation and invasion of several cancers. However, function MALAT1 development thyroid cancer has not been experimentally established. Methods The expression IQGAP1 tissues cells were detected by quantitative real-time PCR western blot. effects on cell with 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium 4 (MTT) assay Transwell assay, respectively. FTC-133 or SW1736 transfected si-MALAT1 pcDNA-MALAT1 injected subcutaneously into 4-week-olds BALB/c mice to examine impact tumor vivo. Results In this study, we discovered higher level MALAT-1 compared control. MTT showed knockdown inhibited. Moreover, could upregulate cells. addition, reversed decreasing induced overexpression. Finally, study vivo verified promoted growth cancer. Conclusion Our via regulating IQGAP1.
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