Lethal effect of recombinant human Fas ligand in mice pretreated with Propionibacterium acnes.

摘要: Fas ligand (FasL) is a type II membrane protein. Binding of FasL to its receptor, Fas, induces apoptosis. Matrix metalloproteinase cleaves the membrane-bound human yield active soluble form. Here, we have produced large amount rFasL using yeast, Pichia pastoris. The purified was found be glycosylated and exist as trimer. effective in inducing apoptosis Fas-expressing T cell or fibroblast line. ID50 for mouse cells about 0.5 ng/ml. killing process with quick. That is, >80% were killed within 1 h by saturation concentration rFasL. Intravenous administration 500 microg had lethal effect mice. When mice pretreated Propionibacterium acnes, subsequent injection 30 induced hepatic failure 24 h. These results indicated that vitro vivo, deleterious may strengthened patients who are suffering from bacterial infection.