Purification of Toxic Saponins from Narthecium asiaticum Maxim..
作者： Masato KOBAYASHI , Kazue SUZUKI , Shigeyoshi NAGASAWA , Yoshihiro MIMAKI
DOI: 10.1292/JVMS.55.401
关键词:
摘要: Toxic substances in Narthecium asiaticum Maxim. were isolated and purified, monitoring the oral toxicity guinea pigs. The crude extract prepared from methanolic of plant contained 2 major saponins (C8 C9), 7 corresponding (C1-7) on TLC. was fractionated by a conventional method for saponin preparation. 1-butanol fraction rich C8 C9, showed toxicity. C9 fractional precipitation silica gel column chromatography. On basis C-13 H-1 nuclear magnetic resonance (NMR) spectral data, acid enzymatic hydrolysis comfirmed to be mixture two furostanol (C9a, C9b); branched trisaccharide composed galactose, glucose, arabinose, linked at 3β-C, glucose 26-C sarsasapogenin smilagenin. considered containing xylose instead arabinose structure C9. Oral administration caused diarrhea, proteinuria, hematuria death
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