New specific markers of human and mouse fibroblasts.

摘要: The expression of different lineage-specific markers was examined on tissue-derived fibroblast cultures in three species (mouse, rat and human), using indirect immunofluorescence or labelled streptavidin-biotin techniques. Smooth muscle cells a human monocyte-like cell line (U 937) were used as controls. Five monoclonal antibodies against epitopes et two mouse fibroblasts selected during this screening cultures. Three surface (Thy-1, 6-19 1B10) and, particularly, one (5B5) cytoplasmic marker suitable for labeling frozen, but not paraffin-embedded, tissue sections. Unfortunately, the appeared difficult limited interest histopathologic strategy.