Deep Imaging: the next frontier in microscopy

摘要: The microscope is the quintessential tool for discovery in cell biology. From its earliest incarnation as a to make unseen visible, microscopes have been at center of most revolutionizing developments biology, histology and pathology. Major quantum leaps imaging involved dramatic improvements resolution see increasingly smaller structures, methods visualize specific molecules inside cells tissues, ability peer into living study dynamics cellular structures. latest revolution microscopy Deep Imaging—the look very large numbers samples by high-throughput high spatial temporal resolution. This approach rooted development fully automated high-resolution application advanced computational image analysis mining methods. Imaging enabling two novel, powerful approaches biology: thousands with optical precision allows every discernible morphological pattern be used read-out large-scale imaging-based screens, particularly conjunction RNAi-based screening technology; addition, capacity capture images, combined methods, has also opened door detect analyze rare events. These applications are