Osteopontin-c splicing isoform contributes to ovarian cancer progression.
作者： Tatiana M Tilli , Vanessa Ferreira Franco , Bruno Kaufmann Robbs , Joao Luiz Mendes Wanderley , Fabrício Ribeiro de Azevedo da Silva
DOI: 10.1158/1541-7786.MCR-10-0463
关键词:
摘要: Ovarian carcinoma is one of the most aggressive gynecological diseases and generally diagnosed at advanced stages. Osteopontin (OPN) proteins overexpressed in ovarian cancer involved tumorigenesis metastasis. Alternative splicing OPN leads to 3 isoforms, OPNa, OPNb, OPNc. However, expression pattern roles each these isoforms have not been previously characterized cancer. Herein, we evaluated profiling tumor nontumor samples their putative biology using vitro vivo functional assays. OPNa OPNb were expressed both samples, whereas OPNc was specifically samples. The isoform significantly activated OvCar-3 cell proliferation, migration, invasion, anchorage-independent growth formation vivo. Additionally, also shown that some OPNc-dependent protumorigenic are mediated by PI3K/Akt signaling pathway. stimulated immortalized epithelial IOSE indicating a role for this tumorigenesis. Functional assays conditioned medium an anti-OPNc antibody cellular effects observed herein promoted secreted According our data, OPNc-specific its on favoring different aspects progression suggest contributes physiopathology Altogether, data open possibilities new therapeutic approaches selectively down regulate OPNc, altering properties progression.
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