Introduction of human chromosome 11 via microcell transfer controls tumorigenic expression of HeLa cells.
作者： P.J. Saxon , E.S. Srivatsan , E.J. Stanbridge
DOI: 10.1002/J.1460-2075.1986.TB04670.X
关键词:
摘要: Both tumorigenic segregant HeLa X human fibroblast hybrids and (D98/AH-2) cells can be converted to a non-tumorigenic state following introduction of single copy t(X;11) chromosome. The translocated chromosome contains approximately 95% the 11 q26-qter portion which hypoxanthine guanine phosphoribosyl transferase (HPRT) gene. Introduction has no effect on expression. Suppression tumorigenicity is relieved by selecting have lost growth in medium containing 6-thioguanine (6-TG). Further, reintroduction into 6TGR again suppresses tumorigenicity. Thus, sufficient completely suppress phenotype suggestive presence tumor-suppressor gene(s) this
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