Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons.

摘要: A complex interplay between multiple chromatin modifiers is critical for cells to regulate structure and accessibility during essential DNA-templated processes such as transcription. However, the coordinated activities of these in regulation gene expression are not fully understood. We previously determined that budding yeast histone H4 methyltransferase Set5 functions together with Set1, H3K4 methyltransferase, specific cellular contexts. Here, we sought understand relationship evolutionarily conserved enzymes expression. generated a comprehensive genetic interaction map functionally uncharacterized expanded existing interactome global modifier revealing functional overlap two chromatin-related networks, Furthermore, profiling via RNA-Seq revealed an unexpected synergistic role Set1 repressing transcription Ty transposable elements genes located subtelomeric regions. This study uncovers novel pathways which participates and, more importantly, reveals partnership transcriptional repression near repetitive DNA yeast. Together, our results define new H3 methyltransferases, whose combined activity may be implicated preserving genomic integrity.