Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast

摘要: Eukaryotic gene expression requires that RNA Polymerase II (RNAP II) gain access to DNA in the context of chromatin. The C-terminal domain (CTD) RNAP recruits chromatin modifying enzymes promoters, allowing for transcription initiation or repression. Specific CTD phosphorylation marks facilitate recruitment modifiers, transcriptional regulators, and processing factors during cycle. However, readable code recruiting such is still not fully defined how modifications affect related families genes regional well understood. Here, we examine effects manipulating Y1S2P3T4S5P6S7 heptapeptide repeat Schizosaccharomyces pombe by substituting non-phosphorylatable alanines Ser2 and/or Ser7 phosphomimetic glutamic acid Ser7. Global analyses were conducted using splicing-sensitive microarrays validated via RT-qPCR. mutations did pre-mRNA splicing snRNA levels. Rather, data revealed upregulation subtelomeric alteration repressive histone H3 lysine 9 methylation (H3K9me) landscape. further indicate H3K9me status are correlated, suggestive CTD-dependent repression mechansims act independently