Polypeptide structure and encoding location of the adenovirus serotype 2 late, nonstructural 33K protein.

摘要: Radiochemical microsequence analysis of selected tryptic peptides the adenovirus type 2 33K nonstructural protein has revealed precise region genomic nucleotide sequence that encodes this protein. The initiation codon for lies 606 nucleotides to right EcoRI restriction site at 70.7 map units and 281 left postulated carboxyterminal 100K coding regions these two proteins thus overlap; however, is derived from +1 frame with respect reading frame. Our results contradict an earlier published report suggesting share extensive amino acid homology (N. Axelrod, Virology 87:366-383, 1978). Ad2 EcoRI-F fragment (70.7 75.9 units) cannot accommodate in a single peptide sequences we have determined. Sequence DNA fragments virus confirmed all critical Consequently, our data are only consistent existence mRNA splice within 33K. Consensus donor acceptor been located would predict removal 202 transcripts Removal explain structure otherwise be directly encoded by fragment. Identification points sequencing permitted prediction complete