Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

摘要: The complete DNA sequence of bovine adenovirus type 3 is reported here. size the genome 34,446 bp in length with a G+C content 54%. All genes early and late regions are present expected locations genome. However, late-region organized into seven families, instead five as they human 2. deduced amino acid sequences open reading frames (ORFs) region 2 (E2) for IVa2 show higher degrees homology, whereas predicted ORFs E1, E3, E4 pIX, fiber, 33,000-molecular-weight nonstructural proteins little or no homology corresponding other adenoviruses. In addition, penton base protein lacks integrin binding motif, RGD, but has an LDV motif MDV motif. Interestingly, animal adenoviruses, virus-associated RNA appear to be absent from their usual location. Sequence analysis cDNA clones representing early- identified splice acceptor donor sites, polyadenylation signals tripartite leader sequences.