A highly sensitive and selective method for the determination of Leukotriene B4 in human plasma by negative ion chemical ionization/gas chromatography/tandem mass spectrometry
作者： Mayumi Takamoto , Tomoko Yano , Tetsuya Shintani , Seiji Hiraku
DOI: 10.1016/0731-7085(95)01583-3
关键词:
摘要: We have developed a highly sensitive and selective method for the determination of Leukotriene B4 (LTB4) in human plasma using negative ion chemical ionization/gas chromatography/tandem mass spectrometry (NICI/GS/MS/MS) analysis. The was summarized as follows. Deuterated LTB4 (d4-LTB4) added to samples an internal standard, were extracted by Sep-pak C18 column. Extracted derivatized into pentafluorobenzyl ester bis-trimethylsilyl ether (PFB-TMS-LTB4) quantified on basis selected reaction monitoring (SRM) at m/z 299 [M-PFB-2TMSOH]- NICI/GC/MS/MS analysis, which product [M-PFB]-. detection limit quantification 10 pg ml-1, sufficiently determine concentrations endogenous plasma. level measured healthy male volunteers 33.85 +/- 33.91 ml-1 (mean S.D. six volunteers). technique MS/MS used this offers much greater sensitivity selectivity than single-stage spectrometry. showed good reproducibility with simple rapid extraction procedure, would be useful examining relationship between various disease states levels biological fluids.
elsevier.com
sci-hub.se 参考文章(10)
Kunihiko Shindo, Kaoru Miyakawa, Motonori Fukumura, Leukotriene B4 levels in the arterial blood of patients with chronic obstructive pulmonary disease Current Therapeutic Research-clinical and Experimental. ,vol. 54, pp. 11- 14 ,(1993) , 10.1016/S0011-393X(05)80612-5
F. Kohi, D.K. Agrawal, J.B. Cheng, A. Bewtra, R.G. Townley, J.W. Olesch, The development of a sensitive and specific radioreceptor assay for leukotriene B4. Life Sciences. ,vol. 42, pp. 2241- 2248 ,(1988) , 10.1016/0024-3205(88)90376-1
Jytte Fogh, Lars K. Poulsen, Hans Bisgaard, A specific asay for lukotriene B4 in human whole blood Journal of Pharmacological and Toxicological Methods. ,vol. 28, pp. 185- 190 ,(1992) , 10.1016/1056-8719(92)90002-I
J. MacDermot, C.R. Kelsey, K.A. Waddell, R. Richmond, R.K. Knight, P.J. Cole, C.T. Dollery, D.N. Landon, I.A. Blair, Synthesis of leukotriene B4, and prostanoids by human alveolar macrophages: analysis by gas chromatography/ mass spectrometry Prostaglandins. ,vol. 27, pp. 163- 179 ,(1984) , 10.1016/0090-6980(84)90071-6
Th. Strasser, H. Schiffl, Leukotriene B4 is involved in hemodialysis-associated neutropenia Biochimica et Biophysica Acta. ,vol. 1046, pp. 326- 329 ,(1990) , 10.1016/0005-2760(90)90249-W
T Simmet, W Luck, Radioreceptor assay for leukotriene B4. Use for determination of leukotriene B4 formation by whole human blood. Eicosanoids. ,vol. 1, pp. 107- 110 ,(1988)
Frank Carey, Robert A. Forder, Radioinmunoassay of LTB4 and 6-trans LTB4: Analytical and pharmacological characterisation of immunoreactive LTB4 in ionophore stimulated human blood Prostaglandins, Leukotrienes and Medicine. ,vol. 22, pp. 57- 70 ,(1986) , 10.1016/0262-1746(86)90022-3
F. Carey, R.A. Forder, K.H. Gibson, D. Haworth, Radioimmunoassay of LTB4 in plasma from different species: a cautionary note. Prostaglandins Leukotrienes and Essential Fatty Acids. ,vol. 36, pp. 57- 61 ,(1989) , 10.1016/0952-3278(89)90163-4
Toshio Ozaki, Hideki Hayashi, Kenji Tani, Fumitaka Ogushi, Susumu Yasuoka, Takeshi Ogura, Neutrophil chemotactic factors in the respiratory tract of patients with chronic airway diseases or idiopathic pulmonary fibrosis. The American review of respiratory disease. ,vol. 145, pp. 85- 91 ,(1992) , 10.1164/AJRCCM/145.1.85
T. Henden, H. Strand, E. Børde, A.G. Semb, T.S. Larsen, Measurements of leukotrienes in human plasma by solid phase extraction and high performance liquid chromatography Prostaglandins Leukotrienes and Essential Fatty Acids. ,vol. 49, pp. 851- 854 ,(1993) , 10.1016/0952-3278(93)90209-F