Increase of Protein Tyrosine Phosphorylation in Rat Retina After Ischemia-Reperfusion Injury
作者： B M Koroma , E de Juan , K Imai , A Hayashi
DOI:
关键词:
摘要: PURPOSE This study was conducted to examine the effect of retinal ischemia-reperfusion injury on protein tyrosine phosphorylation, production angiogenic growth factors, and activation signal proteins in kinase pathways. METHODS Ischemia-reperfusion induced rats by compression optic nerve for 2 hours. The were killed, retinas collected at 0, 1, 6, 24, 48, 96, or 168 hours reperfusion. Tyrosine phosphorylation retina examined Western blot analysis immunohistochemistry. Angiogenic factors their receptors, such as basic fibroblast factor (bFGF) Flg, vascular endothelial (VEGF) Flk-1, platelet-derived (PDGF)-B chain PDGF-beta receptor, five intracellular (phosphatidylinositol 3-kinase [PI3K], phospholipase C gamma [PLC gamma], C-Src, SHC, mitogen-activated [MAPK]) analysis. RESULTS Protein increased after injury, reaching a peak 48 Increased staining tyrosine-phosphorylated inner evident immunohistochemical examination. amount bFGF decreased but amounts VEGF PDGF-B increased. PLC gamma, MAPK reperfusion, receptor PI3K CONCLUSIONS rat leads pathway, increasing factors. resultant MAPK, PI3K, may play an important role ischemia-induced changes cell proliferation.
arvojournals.org参考文章(38)
M Hangai, Y Honda, K Yabuuchi, M Yoshida, N Yoshimura, Interleukin-1 gene expression in transient retinal ischemia in the rat. Investigative Ophthalmology & Visual Science. ,vol. 36, pp. 571- 578 ,(1995)
T Kuwabara, E Stefánsson, C A Wilson, T Schoen, Experimental ischemia induces cell mitosis in the adult rat retina. Investigative Ophthalmology & Visual Science. ,vol. 29, pp. 1050- 1055 ,(1988)
Arnall Patz, Clinical and experimental studies on retinal neovascularization. XXXIX Edward Jackson Memorial Lecture. American Journal of Ophthalmology. ,vol. 94, pp. 715- 743 ,(1982) , 10.1016/0002-9394(82)90297-5
S. V. Nicosia, R. F. Nicosia, M. Smith, Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro. American Journal of Pathology. ,vol. 145, pp. 1023- 1029 ,(1994)
Danqun Guo, Qing Jia, Ho-Yeong Song, Robert S. Warren, David B. Donner, Vascular Endothelial Cell Growth Factor Promotes Tyrosine Phosphorylation of Mediators of Signal Transduction That Contain SH2 Domains Journal of Biological Chemistry. ,vol. 270, pp. 6729- 6733 ,(1995) , 10.1074/JBC.270.12.6729
T. Sasaoka, D.W. Rose, B.H. Jhun, A.R. Saltiel, B. Draznin, J.M. Olefsky, Evidence for a functional role of Shc proteins in mitogenic signaling induced by insulin, insulin-like growth factor-1, and epidermal growth factor. Journal of Biological Chemistry. ,vol. 269, pp. 13689- 13694 ,(1994) , 10.1016/S0021-9258(17)36885-0
I Hemo, A Itin, J Pe'er, E Keshet, D Shweiki, H Gnessin, HYPOXIA-INDUCED EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR BY RETINAL CELLS IS A COMMON FACTOR IN NEOVASCULARIZING OCULAR DISEASES Laboratory Investigation. ,vol. 72, pp. 638- 645 ,(1995)
J Xu, M Nakahara, J.W. Crabb, E Shi, Y Matuo, M Fraser, M Kan, J Hou, W.L. McKeehan, Expression and immunochemical analysis of rat and human fibroblast growth factor receptor (flg) isoforms. Journal of Biological Chemistry. ,vol. 267, pp. 17792- 17803 ,(1992) , 10.1016/S0021-9258(19)37114-5
Brygida Berse, Anthony P. Adamis, Patricia A. D'Amore, Kiang Teck Yeo, Judah Folkman, Joan W. Miller, Lawrence F. Brown, Rachel S. Moulton, Harold F. Dvorak, Michael S. O'Reilly, Tet Kin Yeo, David T. Shima, Vascular endothelial growth factor/vascular permeability factor is temporally and spatially correlated with ocular angiogenesis in a primate model. American Journal of Pathology. ,vol. 145, pp. 574- 584 ,(1994)
M Doly, P Braquet, M E Szabo, C Carré, M T Droy-Lefaix, Ischemia and reperfusion-induced histologic changes in the rat retina. Demonstration of a free radical-mediated mechanism. Investigative Ophthalmology & Visual Science. ,vol. 32, pp. 1471- 1478 ,(1991)