Central Role of p53 on Regulation of Vascular Permeability Factor/Vascular Endothelial Growth Factor (VPF/VEGF) Expression in Mammary Carcinoma
作者： Soumitro Pal , Debabrata Mukhopadhyay , Kaustubh Datta
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摘要: The process of angiogenic switching is one the most important factors in growth and development breast tumors. Vascular permeability factor/vascular endothelial factor (VPF/VEGF) considered to be directly acting protein that has been shown up-regulated cancer cells. Hypoxia seems an stimulus for inducing VPF/VEGF mRNA expression human mammary Here, we have studied roles tumor suppressor gene p53 proto-oncogene c-Src regulating transcription cell lines MCF-7 MDA-MB 435 under both normoxic hypoxic conditions. significantly inhibited involving Sp1. Increased binding Sp1 promoter observed when cells were exposed hypoxia. It makes a complex with inhibits its prevent transcriptional activation. Furthermore, kinase activity was found increased condition, presence antisense Src, there down-regulation total level also VPF/VEGF. present study indicates can inhibit induction Src thereby may transcription. Taken together, our data suggest central role p53, through which it by down-regulating activity,
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