In a Neonatal Piglet Model of Intestinal Failure, Administration of Antibiotics and Lack of Enteral Nutrition Have a Greater Impact on Intestinal Microflora Than Surgical Resection Alone

摘要: Background: Data are limited on how short bowel syndrome (SBS) affects the healthy developing intestinal microbiome, with even less assessing different SBS anatomical surgical models. This study was conducted to describe “ileal” and “colonic” microflora in 2 models of SBS. Materials Methods: Neonatal piglets (2–5 days old) underwent resection, leaving ileum (JI anatomy, n = 6) or removing ileocecal valve (JC 5), sham surgery (sham; 4). JI, JC, commenced parenteral nutrition day 0 received ampicillin trimethoprim-sulfadoxine 0–4 for prevention line sepsis. At 7, ileal colonic digesta were collected, they also collected from age-matched sow-fed (n 6). DNA extraction, sequencing, annotation followed standard procedures. Results: Colonic bacterial genus diversity relative abundance greater (P < .05) compared piglet...