Frontal affinity chromatography: An excellent method of analyzing weak biomolecular interactions based on a unique principle.

作者: Kenichi Kasai

DOI: 10.1016/J.BBAGEN.2020.129761

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摘要: Abstract Background Not only strong biomolecular interactions but also weak play important roles in ensuring appropriate operations of many biological systems. Although a thorough investigation the latter is essential understanding life science, few suitable research tools are available because inherent difficulties. Scope review Frontal affinity chromatography (FAC) versatile method that overcomes difficulties to provide accurate information on interactions. Since its concept and merit not widely recognized, comprehensive interpretation FAC provided this encourage application among researchers. Major conclusion based unique principle measuring binding strength by delayed migration an analyte through column. Its utility was elucidated via lectin-glycan General significance has great potential as tool solve difficult problems general bioscience relevant almost all

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