Interference between inactivated and active influenza viruses in the chick embryo

摘要: Abstract A comparison was made of the interfering capacities several types ultraviolet-irradiated incomplete and standard influenza virus preparations on basis similar hemagglutinating activities. Serial decimal dilutions challenge were employed, results evaluated in terms yields infectious progeny. Incomplete derived from chick embryos by undiluted passage series or injection partially heat-inactivated seed (37° for days) interfered to about same extents as complete virus, long amount internal S antigen did not fall below a certain minimum. Virus components with smaller concentrations S, obtained allantoic membranes third passage, revealed definite, but loss activity. The S-free HA separated after exposure ether failed induced interference, particles infected HeLa cultures. No evidence has been found indicate that latter incorporate antigen, although it is produced cells. These are compatible hypothesis its RNA (ribonucleic acid) part, constitutes actual agent responsible inducing interference. However, per se could be shown interfere. This does necessarily deny role since entry into cells under experimental conditions established. fact two preparations, which possessed full receptor-destroying-enzyme activity, cause reaffirms previous conclusions phenomenon referable receptor mechanism.