The possibilities and challenges of in vitro methods for plant conservation

摘要: Seed-based methods are generally the most efficient for propagating and storing plant germplasm, but these not always adequate, some species can benefit from in vitro conservation. For that produce few or no seeds wild, plants may be propagated vitro, shoot tips provide material cryostorage when available recalcitrant. In also serve as subjects research, without depleting genetic resources of species. Clonal used to test out suitable habitat basic research on endangered species, disturbing wild population. Despite effectiveness widely techniques, however, there still resist initiation into culture difficult root acclimatise. Similarly, tissue cryopreservation restrained by cost, particularly maintaining multiple genotypes many Maintaining such is costly runs risk loss change over time. Examples successful use will illustrate variety applications costs specific challenges discussed help define areas where further needed realise potential a tool