Cockle Shell-Derived Aragonite CaCO3 Nanoparticles for Co-Delivery of Doxorubicin and Thymoquinone Eliminates Cancer Stem Cells.
作者： Kehinde Muibat Ibiyeye , Abu Bakar Zakaria Zuki
DOI: 10.3390/IJMS21051900
关键词:
摘要: Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D enriched spheroids in serum-free conditions evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs free were also evaluated. WST assay, sphere forming ALDH activity analysis, Surface marker CD44 CD24 expression, apoptosis Annexin V-PI kit, cell cycle morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay migration carried out; The combination therapy showed enhanced apoptosis, reduction expression surface maker, cellular invasion, inhibition formation when compared single drug-loaded nanoparticle. Scanning microscopy poor spheroid formation, membrane blebbing, presence shrinkage, distortion architecture; results from this that nanoparticles can efficiently destroy breast nanoparticle simple mixture doxorubicin thymoquinone.
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