Alteration of the Cell Periphery During Granulocyte Maturation: Relationship to Cell Function

摘要: Physiologic characteristics of the surface human granulocytes may be important determinants functional capacity. Studies immature and polymorphonuclear neutrophils (PMN’s) from marrow blood indicated that are characterized by a high, neuraminidase-susceptible, negative charge density; high degree cellular rigidity, as measured an elastimeter with micropipette orifice size approximated estimates average bone basement membrane pore (3.5µ); low adhesiveness to glass plastic; propensity aggregate; slow rate cell spreading, pseudopod extension, motility; very phagocytosis. At myelocyte stage, phagocytosis, although not prominent, became evident. In contrast PMN’s had lower density, higher deformability (the entire PMN readily deforming enter 3.5µ whereas granulocyte would so deform), (plastic > glass), aggregate, formation motility, The increased compared reflection altered relationship between relaxing contracting systems at periphery during maturation and/or changes in sol-gel state macromolecules periphery. This concept is supported change properties toward those cold temperature (4°C). reduced could facilitate and, thereby, increase cell-surface cell-particle contact enhancement motility Hence exit circulation capacity determined process cytoplasmic granulopoiesis adapts for its essential cell-extracell interactions.