Endothelin-1 and endothelin-4 stimulate monocyte production of cytokines.

摘要: Objective To determine the effect of endothelin-1 and endothelin-4 on human monocyte production cytokines. Design Previous work from our laboratory has shown that activates leukocytes. Endothelin-1 endothelin-3 are principally produced by vascular endothelium. However, epidermal cells in gut mucosa, lung, kidney produce endothelin-2 endothelin-4, which differ a single amino acid. While structurally similar to endothelin-1, may affect smooth muscle other tissues differently. The was examined interleukins (IL) neutrophil activation factors. Setting A clinically-oriented basic science Veteran's Administration Hospital Medical Center. Subjects Healthy volunteer adult male/female medical students, researchers, hospital workers. Interventions Human peripheral blood mononuclear were separated density gradients cultured media, with or without addition bacterial endotoxin varying molar concentrations endothelin-4. Supernatants harvested at 10 mins, 1, 6, 12, 24, 48 hrs, enzyme-linked immunosorbent assays performed presence tumor necrosis factor-alpha, IL-1 beta, IL-6, IL-8, granulocyte-macrophage colony-stimulating factor. Measurements Main Results potent stimuli for They also caused beta IL-6 production. Conclusion activate leukocytes after shock ischemia, resulting further injury reperfused distant lungs organs. Crit Care Med 1997; 25:958-964)