Method for analyzing dynamic detectable events at the single molecule level

作者: Anelia Kraltcheva , Nasanshargal Battulga , Costa M. Colbert , Keun Woo Lee , Ivan Pan

DOI:

关键词:

摘要: A method to be implemented on or in a computer is disclosed, where the includes data collection, calibration, candidate selection, and analysis of streams associated with each classify single molecule fluorescence resonance energy transfer events. Once classified, classification can related nature events, such as identification dNTP incorporation during primer extension obtain base read out an unknown template.

参考文章(295)
Charles C. Richardson, Stanley Tabor, Ella Bedford, DNA polymerase with modified processivity ,(1996)
Mark A. Johnson, Rex A. Peterson, Andrew G. Ragatz, Gas flux system chamber design and positioning method ,(2005)
L.R. Yarbrough, J.G. Schlageck, M. Baughman, Synthesis and properties of fluorescent nucleotide substrates for DNA-dependent RNA polymerases. Journal of Biological Chemistry. ,vol. 254, pp. 12069- 12073 ,(1979) , 10.1016/S0021-9258(19)86429-3
Garrick Murray Little, Harry Leonard Osterman, Narasimhachari Narayanan, Ramesh Raghavachari, Fluorescent cyanine dyes ,(1998)
Charles C. Richardson, Stanley Tabor, DNA sequencing with pyrophosphatase ,(1994)
Babetta L. Marrone, James H. Jett, Richard A. Keller, John C. Martin, Richard G. Posner, Daniel J. Simpson, Mark L. Hammond, Method for rapid base sequencing in DNA and RNA with two base labeling ,(1994)
Elana Swartzman, Ronald J. Graham, Lily Lu, Linda G. Lee, William E. Werner, Water-soluble rhodamine dye conjugates ,(2005)