Nanoliposomal Nitroglycerin Exerts Potent Anti-Inflammatory Effects.

摘要: Nitroglycerin (NTG) markedly enhances nitric oxide (NO) bioavailability. However, its ability to mimic the anti-inflammatory properties of NO remains unknown. Here, we examined whether NTG can suppress endothelial cell (EC) activation during inflammation and developed nanoformulation simultaneously amplify effects ameliorate adverse associated with high-dose administration. Our findings reveal that significantly inhibits human U937 adhesion NO-deficient microvascular ECs in vitro through an increase decrease ICAM-1 clustering, as determined by analyzer, microfluorimetry, immunofluorescence staining. Nanoliposomal (NTG-NL) was formulated encapsulating within unilamellar lipid vesicles (DPhPC, POPC, Cholesterol, DHPE-Texas Red at molar ratio 6:2:2:0.2) were ~155 nm diameter readily uptaken ECs, dynamic light scattering quantitative fluorescence microscopy, respectively. More importantly, NTG-NL produced a 70-fold therapeutic efficacy when compared free while preventing excessive mitochondrial superoxide production high doses. Thus, these findings, which are first superior nanoformulation, provide rationale for their detailed investigation potentially vascular normalization therapies.