Effect of acidotic blood reperfusion on reperfusion injury after coronary artery occlusion in the dog heart.

摘要: A prolongation of the intracellular acidosis after myocardial ischemia can protect myocardium against reperfusion injury. In isolated hearts, this was achieved by extracellular acidosis. The aim study to investigate whether regional with acidotic blood coronary artery occlusion reduce infarct size and improve function in vivo. Anesthetized open-chest dogs were instrumented for measurement function, assessed sonomicrometry as systolic wall thickening (sWT). Infarct determined triphenyltetrazolium staining 3 h reperfusion. left anterior descending (LAD) perfused through a bypass from carotid artery. animals underwent 1 LAD subsequent bypass-reperfusion normal (control, n = 6) or equilibrated pH 6.8 using 0.1 mM HCl during first 30 min (HCl, 5). Regional collateral flow (RCBF) at 30-min measured colored microspheres. There no difference recovery sWT LAD-perfused area between two groups end experiments [-2.8+/-1.2% (HCl) vs. -4.4+/-2.5% (control); mean +/- SEM; p NS]. RCBF comparable both groups. (percentage risk) reduced treatment group (12.8+/-2.8%) compared control (26.2+/-4.8%; < 0.05). These results indicate that injury be prolonged local