Exercise rescues the immune response fine-tuned impaired by peroxisome proliferator-activated receptors γ deletion in macrophages.

摘要: Background Exercise is a powerful tool for prevention and treatment of many conditions related to the cardiovascular system also chronic low-grade inflammation. Peroxisome proliferator-activated receptors γ (PPARγ) exerts an import role on regulation metabolic profile subsequent inflammatory response, especially in macrophages. Purpose To investigate effects 8-week moderate-exercise training parameters mice with PPARγ deficiency myeloid cells. Methods Twelve-week old bearing deletion exclusively cells (PPARγlox/lox Lys Cre -/+ , knockout [KO]) littermate controls -/- wild type [WT]) were submitted exercise (treadmill running at moderate intensity, 5 days/week). Animals evaluated food intake, glucose homeostasis, serum metabolites, adipose tissue peritoneal macrophage inflammation, basal stimulated cytokine secretion. Results protocol did not improve metabolism or adiponectin concentrations KO mice. Moreover, absence macrophages exacerbated proinflammatory sedentary Peritoneal cultured had higher tumor necrosis factor-α (TNF-α) secretion nonstimulated lipopolysaccharide (LPS)-stimulated Toll-4 receptor (TLR4) gene expression under LPS stimulus. Trained showed reduced TNF-α content independently genotype. M2 polarization ability was impaired after training, while tissue-associated present any effect by ablation. Conclusion Overall, seems necessary maintain appropriate response stimulus polarization, affecting whole body lipid profile. as efficient mechanism restore immune