Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors.
作者： N Hussy , W Lukas , K A Jones
DOI: 10.1113/JPHYSIOL.1994.SP020441
关键词:
摘要: 1. A comparative study of the whole-cell and single-channel properties cloned native mouse 5-hydroxytryptamine ionotropic receptors (5-HT3) was undertaken using mammalian cell lines expressing 5-HT3 receptor subunit (5-HT3R-A), superior cervical ganglia (SCG) neurones N1E-115 cells. 2. No pharmacological difference found in sensitivity to agonists 5-HT 2-methyl-5-HT, or antagonists d-tubocurare 3-tropanyl-3,5-dichlorobenzoate (MDL-72222). 3. Current-voltage (I-V) relationships currents showed inward rectification three preparations. Rectification stronger both cells 5-HT3R-A when compared with SCG neurones. 4. clear openings could be resolved 5-HT-activated patches excised from Current fluctuation analysis excised-patch records revealed a slope conductance 0.4-0.6 pS these channels strong that fully accounted for voltage dependence. 5. In contrast, single about 10 were activated by The weak dependence their did not account completely currents. lower unitary (3.4 pS) inferred noise analysis. 6. We conclude expressed cDNA is indistinguishable cells, suggesting an identical structure two receptors. higher different might indicate participation additional ganglionic Homo-oligomeric may also present as suggested estimated
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