Identification of novel genes associated with astrocytoma progression using suppression subtractive hybridization and real‐time reverse transcription‐polymerase chain reaction

摘要: To identify novel genes involved in glioma progression we performed suppression subtractive hybridization combined with cDNA array analysis on 4 patients primary low-grade gliomas of World Health Organization (WHO) grade II that recurred as secondary glioblastomas (WHO IV). Eight showing differential expression between and recurrent tumors 3 the were selected for further using real-time reverse transcription-PCR a series 10 pairs high-grade well 42 astrocytic different WHO grades. These analyses revealed 5 genes, i.e., AMOG (ATP1B2, 17p13.1), APOD (3q26.2-qter), DMXL1 (5q23.1) DRR1 (TU3A, 3p14.2) PSD3 (KIAA09428/HCA67/EFA6R, 8p22), expressed at significantly lower levels compared to diffuse astrocytomas II. In addition, AMOG, transcript than astrocytomas. Treatment cell lines 5-aza-2'-deoxycytidine trichostatin A resulted increased transcripts. Sequencing sodium bisulfite-modified DNA demonstrated promoter hypermethylation 1 anaplastic astrocytoma low expression. Taken together, identified interesting candidate likely contribute provide first evidence role epigenetic silencing malignant cells.