Sampling for airborne influenza virus using RNA preservation buffer

摘要: Characterizing airborne influenza virus exposure is important for infection prevention and control in health care public settings. Detecting assessing risk. The must also be protected from deterioration during aerosol sampling long term storage. RNA preservation buffers (RNAPBs) may stabilize after Bioaerosol samplers are used to collect virus, many different types of available. objectives this experiment were to: 1) compare concentrations across bioaerosol samplers; 2) the efficacy RNAPB over Hanks Balanced Salt Solution (HBSS) as a sample collection media; 3) determine whether stabilizes viral particles stored time. In we aerosolized active (H1N1) chamber compared efficiencies using two samplers: SKC Biosampler NIOSH Biosampler, medias: an RNAPB. Ten 15-minute experimental trials completed. We HBSS terms maintenance integrity storage at room temperatures. Samples temperature 1, 4, 7, 14 days. Virus measured each time point. Significant differences found between sampler media type – collected higher concentration than conditions, maintained significantly greater