Antiangiogenesis Is Produced by Nontoxic Doses of Vinblastine

摘要: The effects of vinblastine (VBL) on endothelial cell functions involved in angiogenesis, namely proliferation, chemotaxis, spreading fibronectin (FN), secretion matrix-metalloproteinase-2 (MMP-2) and MMP-9, morphogenesis Matrigel were tested vitro, whereas its angiogenesis studied vivo by using the chick embryo chorioallantoic membrane (CAM) model. In at noncytotoxic doses (0.1, 0.25, 0. 5, 0.75, 1 pmol/L), VBL impacted all these functions, except MMPs, a dose-dependent fashion. By contrast, proliferation other primary cells such as fibroblasts lymphoid tumor was not impacted. vivo, 0.5, pmol/L again displayed antiangiogenic activity. Lack cytotoxicity vitro shown both morphologically, also because rapidly abolished when removed. Apoptosis induced. At ultrastructural level, impairment associated with thin disturbance cytoskeleton, form slight depolymerization accumulation microfilaments, which equally reversible. Results suggest that has an component very low, doses, antiangiogenesis could be used to treat wide spectrum angiogenesis-dependent diseases, including certain chronic inflammatory Kaposi's sarcoma, cancer.