Structure of the Complex Monolayer of Gemini Surfactant and DNA at the Air/Water Interface
作者： Qibin Chen , Xueli Kang , Rong Li , Xuezhong Du , Yazhuo Shang
DOI: 10.1021/LA204089U
关键词:
摘要: The properties of the complex monolayers composed cationic gemini surfactants, [C(18)H(37)(CH(3))(2)N(+)-(CH(2))(s)-N(+)(CH(3))(2)C(18)H(37)],2Br(-) (18-s-18 with s = 3, 4, 6, 8, 10 and 12), ds-DNA or ss-DNA at air/water interface were in situ studied by surface pressure-area per molecule (π-A) isotherm measurement infrared reflection absorption spectroscopy (IRRAS). corresponding Langmuir-Blodgett (LB) films also investigated atomic force microscopy (AFM), Fourier transform (FT-IR), circular dichroism (CD). π-A isotherms AFM images reveal that spacer surfactant has a significant effect on monolayers. As ≤ gemini/ds-DNA can both laterally normally aggregate to form fibril structures heights 2.0-7.0 nm widths from several tens ~300 nm. > they condense platform structure about 1.4 height. Nevertheless, FT-IR, IRRAS, CD spectra, as well images, suggest DNA retains its double-stranded character when complexed. This is very important meaningful for gene therapy because it crucial maintain extracellular genes undamaged obtain high transfection efficiency. In addition, experience transition helical typical ψ-phase supramolecular chiral order.
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sci-hub.se 参考文章(67)
Abraham Ulman, An Introduction to Ultrathin Organic Films: From Langmuir--Blodgett to Self--Assembly ,(1991)
Yasutaka Matsuo, Kuniharu Ijiro, Masatsugu Shimomura, Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir–Blodgett method Colloids and Surfaces B: Biointerfaces. ,vol. 40, pp. 123- 126 ,(2005) , 10.1016/J.COLSURFB.2004.10.025
D. McLoughlin, R. Dias, B. Lindman, M. Cardenas, T. Nylander, K. Dawson, M. Miguel, D. Langevin, Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions. Langmuir. ,vol. 21, pp. 1900- 1907 ,(2005) , 10.1021/LA047700S
Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh, Studies on the Formation of DNA−Cationic Lipid Composite Films and DNA Hybridization in the Composites Journal of Physical Chemistry B. ,vol. 105, pp. 4409- 4414 ,(2001) , 10.1021/JP0034877
Marc D. Porter, Thomas B. Bright, David L. Allara, Christopher E. D. Chidsey, Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistry Journal of the American Chemical Society. ,vol. 109, pp. 3559- 3568 ,(1987) , 10.1021/JA00246A011
Xiaoyan Tang, Thomas Schneider, Daniel A. Buttry, A Vibrational Spectroscopic Study of the Structure of Electroactive Self-Assembled Monolayers of Viologen Derivatives Langmuir. ,vol. 10, pp. 2235- 2240 ,(1993) , 10.1021/LA00019A034
G.B. Sukhorukov, L.A. Feigin, M.M. Montrel, B.I. Sukhorukov, X-ray and infrared study of Langmuir-Blodgett films of the complexes between nucleic acids and aliphatic amines Thin Solid Films. ,vol. 259, pp. 79- 84 ,(1995) , 10.1016/0040-6090(94)06414-8
Yoshio Okahata, Takuya Kobayashi, Kentaro Tanaka, Orientation of DNA Double Strands in a Langmuir−Blodgett Film Langmuir. ,vol. 12, pp. 1326- 1330 ,(1996) , 10.1021/LA950529N
Hang Sun, Wen Li, Lixin Wu, Honeycomb-patterned films fabricated by self-organization of DNA-surfactant complexes. Langmuir. ,vol. 25, pp. 10466- 10472 ,(2009) , 10.1021/LA900322D
Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh, Hybridization of DNA by Sequential Immobilization of Oligonucleotides at the Air−Water Interface Langmuir. ,vol. 16, pp. 9142- 9146 ,(2000) , 10.1021/LA000753Z