Chip-nanoelectrospray quadrupole time-of-flight tandem mass spectrometry of meningioma gangliosides: a preliminary study.

摘要: A strategy combining high-performance thin layer chromatography (HPTLC), laser densitometry, and fully automated chip-based nanoelectrospray (nanoESIchip) performed on a NanoMate robot coupled to QTOF-MS was developed, optimized, for the first time applied mapping structural identification of gangliosides (GGs) extracted purified from human angioblastic meningioma specimen. While HPTLC pattern indicated only seven fractions migrating as GM3, GM2, GM1, GD3, GD1a (nLD1, LD1), GD1b, GT1b, possibly GD2, due high sensitivity, mass accuracy, ability ionize minor species in complex mixtures, nanoESIchip-QTOF MS able discover significantly more GG than ever reported meningioma. Thirty-four distinct glycosphingolipid components which five asialo, one GM4, nine two six GD1 differing their ceramide compositions were identified. All structures presented long-chain bases with 18 carbon atoms, while length fatty acid found vary C11 C25. screening results also that diversity expressed GM1 is higher expected view low proportions evidenced by densitometric quantification. Simultaneous fragmentation meningioma-associated (d18:1/24:1) (d18:1/24:0) MS/MS using CID confirmed postulated moieties provided data glycan core, document each forms both GM1a GM1b isomers are investigated tissue.