Electroporation-mediated improvement of plant regeneration from colt cherry (Prunus avium × pseudocerasus) protoplasts

作者: S.J. Ochatt , P.K. Chand , E.L. Rech , M.R. Davey , J.B. Power

DOI: 10.1016/0168-9452(88)90096-9

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摘要: Abstract Results are presented that show a promotory carry-over effect, of an electroporation treatment isolated cell suspension protoplasts Colt cherry ( Prunus avium × pseudocerasus ), on the growth protoplast-derived calli and plant regeneration capacity. Callus from subjected to three successive exponential pulses at 250 V or 500 showed largest fresh weight increases between subcultures, also exhibited highest frequency based number shoots per callus. These shoots, in turn, produced more prolific root system when compared those derived non-electropulsed protoplasts.

参考文章(17)
E. Neumann, M. Schaefer-Ridder, Y. Wang, P.H. Hofschneider, Gene transfer into mouse lyoma cells by electroporation in high electric fields. The EMBO Journal. ,vol. 1, pp. 841- 845 ,(1982) , 10.1002/J.1460-2075.1982.TB01257.X
A.J.H. Sale, W.A. Hamilton, Effects of high electric fields on micro-organisms. 3. Lysis of erythrocytes and protoplasts. Biochimica et Biophysica Acta. ,vol. 163, pp. 37- 43 ,(1968) , 10.1016/0005-2736(68)90030-8
E. L. Rech, S. J. Ochatt, P. K. Chand, J. B. Power, M. R. Davey, Electro-enhancement of division of plant protoplast-derived cells Protoplasma. ,vol. 141, pp. 169- 176 ,(1987) , 10.1007/BF01272899
Kazuhiko Kinosita, Tian Yow Tsong, Voltage-induced pore formation and hemolysis of human erythrocytes Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 471, pp. 227- 242 ,(1977) , 10.1016/0005-2736(77)90252-8
K. S. Rathore, A. Goldsworthy, Electrical Control of Shoot Regeneration in Plant Tissue Cultures Nature Biotechnology. ,vol. 3, pp. 1107- 1109 ,(1985) , 10.1038/NBT1285-1107
Michael E. Fromm, Loverine P. Taylor, Virginia Walbot, Stable transformation of maize after gene transfer by electroporation Nature. ,vol. 319, pp. 791- 793 ,(1986) , 10.1038/319791A0
S.J. Ochatt, E.C. Cocking, J.B. Power, Isolation, culture and plant regeneration of colt cherry (Prunus avium × pseudocerasus) protoplasts Plant Science. ,vol. 50, pp. 139- 143 ,(1987) , 10.1016/0168-9452(87)90150-6
Adrian J. Cutler, Mohammed Saleem, Permeabilizing Soybean Protoplasts to Macromolecules Using Electroporation and Hypotonic Shock Plant Physiology. ,vol. 83, pp. 24- 28 ,(1987) , 10.1104/PP.83.1.24
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X