The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function.

摘要: Summary Morphogenesis protein C (MorC) of Aggregatibacter actinomycetemcomitans is important for maintaining the membrane morphology and integrity cell envelope this oral pathogen. The MorC sequence operon organization were found to be conserved in Gammaproteobacteria, based on a bioinformatic analysis 435 sequences from representative organisms. Functional conservation was investigated using an A. actinomycetemcomitans morC mutant as model system express homologs four phylogenetically diverse representatives Gammaproteobacteria: Haemophilus influenzae, Escherichia coli, Pseudomonas aeruginosa, Moraxella catarrhalis. strains expressing homologous proteins assessed sensitivity bile salts, leukotoxin secretion, autoaggregation morphology. most closely related organism (H. influenzae) functionally identical A. actinomycetemcomitans. However, genes more distantly organisms restored some but not all phenotypes. In addition, deletion mutagenesis indicated that portion protein, C-terminus DUF490 domain, necessary maintain membrane. Deletion last 10 amino acids domain sufficient disrupt stability secretion. data suggest across Gammaproteobacteria essential physiology.