Crucial importance of PKC-β(I) in LFA-1–mediated locomotion of activated T cells
作者： Y. Volkov , A. Long , S. McGrath , D. Ni Eidhin , D. Kelleher
DOI: 10.1038/88700
关键词:
摘要: Crawling T cell locomotion in which activated lymphocyte function–associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C-β (PKC-β) isoenzyme to microtubule cytoskeleton. In normal cells and lymphoma lines, this type motility accompanied by PKC-β–sensitive cytoskeletal rearrangements formation trailing extensions, are supported microtubules. Expression PKC-β(I) enhanced green fluorescent (EGFP) nonmotile PKC-β–deficient restored their locomotory behavior response a triggering stimulus delivered via LFA-1 correlated directly degree polarization. We have also shown that component tubulin-enriched LFA-1–cytoskeletal complex assembled upon cross-linking. These observations may physiological equivalents at advanced (post-integrin activation) stages extravasation.
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