Variation in Sulfur and Selenium Accumulation Is Controlled by Naturally Occurring Isoforms of the Key Sulfur Assimilation Enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the Arabidopsis Species Range

摘要: Natural variation allows the investigation of both fundamental functions genes and their role in local adaptation. As one essential macronutrients, sulfur is vital for plant growth development also crop yield quality. Selenium are assimilated by same process, although plants do not require selenium, plant-based selenium an important source this element animals. Here, we report use linkage mapping synthetic F2 populations complementation to investigate genetic architecture total leaf concentrations a diverse set Arabidopsis (Arabidopsis thaliana) accessions. We identify accessions collected from Sweden Czech Republic two variants enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 (APR2) with strongly diminished catalytic capacity. APR2 key sulfate selenate reduction, its reduced activity loss-of-function allele apr2-1 Hodonin Shahdara leads lowering flux into compounds, cysteine glutathione, proteins, concomitant increase accumulation leaves. conclude our observation, previously identified weak accession Tadjikistan, that capacity varies 4 orders magnitude across species range, driving significant differences metabolism. The selective benefit, if any, large remains be explored.