Brain-specific receptor-type protein-tyrosine phosphatase RPTP beta is a chondroitin sulfate proteoglycan in vivo.
作者： Hidekazu Yamada , Motoyuki Shimonaka , Yu Yamaguchi , Kenya Shitara , Ken Watanabe
DOI: 10.1016/S0021-9258(17)32144-0
关键词:
摘要: Abstract By using polyclonal antiserum, which recognizes multiple proteoglycan core proteins, we isolated a cDNA species for an unknown chondroitin sulfate in bovine brain. Unexpectedly, DNA sequencing revealed that the encodes open reading frame highly homologous to human receptor-type protein-tyrosine phosphatase, RPTP beta. To prove beta is proteoglycan, raised three antibodies against extracellular and cytoplasmic domains of These have been shown react with smear band ranging from 350 500 kDa brain extracts. Digestion chondroitinase ABC eliminated this gave rise 310/300-kDa doublet was not detected without digestion, indicating almost all molecules contain chains. In cerebellum, immunofluorescence staining chondroitinase-treated sections pericellular localization external internal granular layers. data establish expressed constitutively as brain, suggest sulfates may be essential component physiological function vivo.
sci-hub.st 参考文章(25)
H. Yamada, K. Watanabe, M. Shimonaka, Y. Yamaguchi, Molecular cloning of brevican, a novel brain proteoglycan of the aggrecan/versican family. Journal of Biological Chemistry. ,vol. 269, pp. 10119- 10126 ,(1994) , 10.1016/S0021-9258(17)36998-3
J Shioi, J.P. Anderson, J.A. Ripellino, N.K. Robakis, Chondroitin sulfate proteoglycan form of the Alzheimer's beta-amyloid precursor. Journal of Biological Chemistry. ,vol. 267, pp. 13819- 13822 ,(1992) , 10.1016/S0021-9258(19)49641-5
J.B. Levy, P.D. Canoll, O. Silvennoinen, G. Barnea, B. Morse, A.M. Honegger, J.T. Huang, L.A. Cannizzaro, S.H. Park, T. Druck, The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system. Journal of Biological Chemistry. ,vol. 268, pp. 10573- 10581 ,(1993) , 10.1016/S0021-9258(18)82237-2
M.F. Gebbink, G.C. Zondag, R.W. Wubbolts, R.L. Beijersbergen, I. van Etten, W.H. Moolenaar, Cell-cell adhesion mediated by a receptor-like protein tyrosine phosphatase Journal of Biological Chemistry. ,vol. 268, pp. 16101- 16104 ,(1993) , 10.1016/S0021-9258(19)85392-9
R. K. Margolis, R. U. Margolis, Nervous tissue proteoglycans. Cellular and Molecular Life Sciences. ,vol. 49, pp. 429- 446 ,(1993) , 10.1007/BF01923587
Roberto Perris, Staffan Johansson, Inhibition of neural crest cell migration by aggregating chondroitin sulfate proteoglycans is mediated by their hyaluronan-binding region. Developmental Biology. ,vol. 137, pp. 1- 12 ,(1990) , 10.1016/0012-1606(90)90002-Z
Robert D. Moir, Ralph N. Martins, Ashley I. Bush, David H. Small, Elizabeth A. Milward, Baden A. Rumble, Gerd Multhaup, Konrad Beyreuther, Colin L. Masters, Human Brain βA4 Amyloid Protein Precursor of Alzheimer's Disease: Purification and Partial Characterization Journal of Neurochemistry. ,vol. 59, pp. 1490- 1498 ,(1992) , 10.1111/J.1471-4159.1992.TB08465.X
P. Brittis, D. Canning, J Silver, Chondroitin sulfate as a regulator of neuronal patterning in the retina Science. ,vol. 255, pp. 733- 736 ,(1992) , 10.1126/SCIENCE.1738848
A J Sant, S E Cullen, K S Giacoletto, B D Schwartz, Invariant chain is the core protein of the Ia-associated chondroitin sulfate proteoglycan. Journal of Experimental Medicine. ,vol. 162, pp. 1916- 1934 ,(1985) , 10.1084/JEM.162.6.1916
E. Fischer, H Charbonneau, N. Tonks, Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes Science. ,vol. 253, pp. 401- 406 ,(1991) , 10.1126/SCIENCE.1650499