Control of polyadenylation and alternative splicing of transcripts from adjacent genes in a procyclin expression site: a dual role for polypyrimidine tracts in trypanosomes?

摘要: The procyclin-associated genes (PAGs) of Trypanosoma brucei are located downstream tandemly repeated procyclin and belong to the same alpha-amanitin-resistant polycistronic transcription units. In procyclic form trypanosomes PAG 1 pre-mRNA is alternatively spliced give rise three transcripts 2.7 kb, 1.8 kb 1.3 kb. two larger contain additional short open reading frames (ORFs) upstream major ORF. Trans-splicing generate these occurs different polypyrimidine tracts. A minor population mRNAs also generated by alternative splicing at a tract that begins 524 bp splice acceptor site beta-gene. required for accurate polyadenylation from alpha-gene (1). Alternatively polyadenylated forms can be detected. All sites found similar distance splice-acceptor sites, in each case with between them. Our results point dual role tracts maturation trypanosome mRNAs.